Development of an immunochromatographic assay for detection of Aflatoxin B1 in corn and rice
Keywords:
Aflatoxin B1, immunochromatographic assay, magnetic nanoparticles, sensitivityAbstract
Introduction: Aflatoxin B1 is a toxic contaminant, a threat to human health. Its presence in food was common. Rapid and efficient determination of AFB1 in corn and rice is critical for food safety monitoring.
Objectives: To develop a highly sensitive sandwich immunochromatographic assay for the detection of aflatoxin B1, with greater sensitivity than the strip test.
Methods: During sample preparation, aflatoxin B1 bound to bovine serum albumin to form AFB1-BSA conjugates, captured by both the anti-BSA antibody on the conjugate pad and the anti-AFB1 antibody on the test line, forming a sandwich structure. Key parameters were optimized to achieve the highest sensitivity of the test strip. Optimal parameters include: saturation of the antibody coating on magnetic nanoparticles, suitable nitrocellulose membrane, and optimal antibody concentration on the test line.
Results: Test strips were developed for the rapid detection of aflatoxin B1 in maize and rice. Detection limits: 1.0 µg/L for aflatoxin B1 in the assay buffer and 1.6 µg/L for aflatoxin B1 in spiked maize and rice samples. Repeatability: 5/5 for 5 aflatoxin B1 concentrations analyzed; test strip stability was 6 months when stored at 4°C and 25°C.
Conclusion: The immunochromatographic assay provides an efficient and highly sensitive approach for the in situ detection of AFB1 in maize and rice.
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References
1. Caceres I, Al Khoury A, El Khoury R, Lorber S, Oswald I, El Khoury A, et al. Aflatoxin biosynthesis and genetic regulation: A review [Internet]. Toxins. 2020; 12(3):150. DOI: 10.3390/toxins12030150
2. IARC. Some traditional herbal medicines, some mycotoxins, naphthalene and styrene [Internet]. World Health Organization. 2002 [accesss: 27/11/2024]. Available at: https://publications.iarc.fr/Book-And-Report-Series/Iarc-Monographs-On-The-Identification-Of-Carcinogenic-Hazards-To-Humans/Some-Traditional-Herbal-Medicines-Some-Mycotoxins-Naphthalene-And-Styrene-2002
3. Singh J, Mehta A. Rapid and sensitive detection of mycotoxins by advanced and emerging analytical methods: A review [Internet]. Food science & nutrition. 2020; 8(5):2183-2204. DOI: 10.1002/fsn3.1474
4. Sun X, Chang J, Dong Q, Wang H, Zhang X, Li D, et al. Facile synthesis of Fe3O4@ polyethylenimine with peroxidase-like activity for highly sensitive detection of interferon a-2b [Internet]. Science China Materials. 2023; 66(10):4121-30. DOI: 10.1007/s40843-023-2549-3
5. Wang Y, Jiang J, Fotina H, Zhang H, Chen J. Advances in antibody preparation techniques for immunoassays of total aflatoxin in food [Internet]. Molecules. 2020; 25(18):4113. DOI: 10.3390/molecules25184113
6. QCVN 8-1:2011/BYT, National technical regulation on the limits of mycotoxin contamination in food [Internet]. Vietnam, Hanoi; 2011. [access 27/11/2024]. Available at https://vbpl.vn/FileData/TW/Lists/vbpq/Attachments/26964/VanBanGoc_02_2011_TT-BYT.pdf
7. Zhou Y, Pan F-G, Li Y-S, Zhang Y-Y, Zhang J-H, Lu S-Y, et al. Colloidal gold probe-based immunochromatographic assay for the rapid detection of brevetoxins in fishery product samples [Internet]. Biosensors and Bioelectronics. 2009; 24(8):2744-47. DOI: 10.1016/j.bios.2009.01.034
8. Liu B-H, Hsu Y-T, Lu C-C, Yu F-Y. Detecting aflatoxin B1 in foods and feeds by using sensitive rapid enzyme-linked immunosorbent assay and gold nanoparticle immunochromatographic strip [Internet]. Food Control. 2013; 30(1):184-9. DOI: 10.1016/j.foodcont.2012.07.008
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Copyright (c) 2025 Tien Duc Chu, Trang Thi Thu Nguyen, Anh Tuan Le, Truong Thi Hoang, Chuyen Van Nguyen, Trung Hoang Nguyen, Hoa Thi Vu, Dat Trong Nguyen, Ngoc Thi Minh Nguyen, Tuan Ngoc Nguyen, Ba Van Nguyen
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