Antibacterial activity of blends of essential oils of Minthostachys setosa and Citrus maxima

Authors

Keywords:

Citrus maxima; essential oil; mixture; Minthostachys setosa; synergism

Abstract

Introduction: The increasing resistance to classical antibacterials represents a serious concern in the treatment of infectious diseases. This has led to a need to study new antibacterial molecules, among which essential oils stand out.

Objective: To quantify the main chemical components of the essential oils of Minthostachys setosa and Citrus maxima, establish their organoleptic and physicochemical characteristics, and determine the antibacterial activity of their mixtures.

Methods: The chemical components were quantified using gas chromatography coupled to mass spectrometry. Organoleptic and physicochemical characteristics were determined using standardized methods from the Association of Official Analytical Collaboration (AOAC). Antibacterial activity was determined by the disk diffusion technique.

Results: Pulegone (80.4%) and D-limonene (33.61%) were the main components of the essential oils of M. setosa and C. maxima. The organoleptic characteristics were an intense menthol odor and spicy taste for M. setosa, and a potent citrus odor and slightly bitter taste for C. maxima. The physicochemical characteristics were pH 5.2 and 5.4; densities of 0.914 g/mL and 0.821 mg/mL; and refractive indices of 1.4856 and 1.4739. Mixture 8 (94% M. setosa and 6% C. maxima) showed inhibition zones of 14.77 mm against Pseudomonas aeruginosa and 12.83 mm against Streptococcus mutans.

Conclusions: Pulegone and D-limonene predominated in M. setosa and C. maxima respectively. The organoleptic and physicochemical characteristics correspond to the essential oils studied. The mixture 8 showed antibacterial activity against P. aeruginosa and S. mutans.

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References

1. De Oliveira DMP, Forde BM, Kidd TJ, Harris PN, Schembri MA, Beatson SA, et al. Antimicrobial resistance in ESKAPE pathogens [Internet]. Clin Microbiol Rev. 2020;33(3):e00181. DOI: 10.1128/cmr.00181-19

2. Khameneh B, Iranshahy M, Soheili V, Fazly Bazzaz BS. Review on plant antimicrobials: a mechanistic viewpoint [Internet]. Antimicrob Resist Infect Control. 2019;8:1–28. DOI: 10.1186/s13756-019-0559-6

3. Carpena M, Nuñez-Estevez B, Soria-Lopez A, Garcia-Oliveira P, Prieto MA. Essential oils and their application on active packaging systems: A review [Internet]. Resources. 2021;10(1):7. DOI: 10.3390/resources10010007

4. Rossi C, Chaves-López C, Serio A, Casaccia M, Maggio F, Paparella A. Effectiveness and mechanisms of essential oils for biofilm control on food-contact surfaces: An updated review [Internet]. Crit Rev Food Sci Nutr. 2022;62(8):2172–91. DOI: 10.1080/10408398.2020.1851169

5. Reichling J. Anti-biofilm and virulence factor-reducing activities of essential oils and oil components as a possible option for bacterial infection control [Internet]. Planta Med. 2020;86(8):520–37. DOI: 10.1055/a-1147-4671

6. Carradori S, Di Giacomo N, Lobefalo M, Luisi G, Campestre C, Sisto F. Biofilm and quorum sensing inhibitors: The road so far [Internet]. Expert Opin Ther Pat. 2020;30(12):917–30. DOI: 10.1080/13543776.2020.1830059

7. AOAC. Official Methods of Analysis. 18th ed. Gaithersburg, MD: Association of Official Analytical Chemists; 2006.

8. Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method [Internet]. Am J Clin Pathol. 1996;45(4_ts):493–6. DOI: 10.1093/ajcp/45.4_ts.493

9. Saïdana D, Mahjoub S, Boussaada O, Chriaa J, Mahjoub MA, Chéraif I, et al. Antibacterial and antifungal activities of the essential oils of two saltcedar species from Tunisia [Internet]. J Am Oil Chem Soc. 2008;85(9):817–26. DOI: 10.1007/s11746-008-1251-9

10. Senatore F. Volatile constituents of Minthostachys setosa (Briq.) Epl. (Lamiaceae) from Peru [Internet]. Flavour Fragr J. 1998;13(4):263–5. DOI: 10.1002/(sici)1099-1026(1998070)13:4<263::aid-ffj738>3.0.co;2-h

11. Oualdi I, Elfazazi K, Azzouzi H, Oussaid A, Touzani R. Chemical composition and antimicrobial properties of Moroccan Mentha pulegium L. essential oil [Internet]. Mater Today Proc. 2023;72:3768–74. DOI: 10.1016/j.matpr.2022.09.318

12. Visakh NU, Pathrose B, Narayanankutty A, Alfarhan A, Ramesh V. Utilization of Pomelo (Citrus maxima) Peel Waste into Bioactive Essential Oils: Chemical Composition and Insecticidal Properties [Internet]. Insects. 2022;13:480. DOI: 10.3390/insects13050480

13. Badalamenti N, Bruno M, Schicchi R, Geraci A, Leporini M, Tundis R, et al. Reuse of food waste: The chemical composition and health properties of pomelo (Citrus maxima) cultivar essential oils [Internet]. Molecules. 2022;27(10):3273. DOI: 10.3390/molecules27103273

14. Hardjono H, Dewi EN, Lusiani CE, Febriansyah I, Bachtiar RI. D-limonene from orange (Citrus maxima) peel extraction as destructive agent of styrofoam waste [Internet]. IOP Conf Ser Mater Sci Eng. 2021; 1073(1):012013. DOI:10.1088/1757-899X/1073/1/012013

15. Bhowmik P, Modi B, Roy P, Chowdhury A. Strategies to combat Gram-negative bacterial resistance to conventional antibacterial drugs: a review [Internet]. Osong Public Health Res Perspect. 2023;14(5):333–46. DOI: 10.24171/j.phrp.2022.0323

16. Mariod AA. Effect of Essential Oils on Organoleptic (Smell, Taste, and Texture) Properties of Food. In: Essential Oils in Food Preservation, Flavor and Safety [Internet]. 2016;131–7. DOI: 10.1016/b978-0-12-416641-7.00013-4

17. Bunse M, Daniels R, Gründemann C, Heilmann J, Kammerer DR, Keusgen M, et al. Essential Oils as Multicomponent Mixtures and Their Potential for Human Health and WellBeing [Internet]. Front Pharmacol. 2022;13:956541. DOI: 10.3389/fphar.2022.956541

18. Mahboubi M, Haghi G. Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil [Internet]. J Ethnopharmacol. 2008;119(2):325–7. DOI: 10.1016/j.jep.2008.07.023

19. Abu-Hussien SH, Nasry AR, Samy Z, El-Sayed SM, Bakry A, Ebeed N, et al. Synergistic antimicrobial activity of essential oils mixture of Moringa oleifera, Cinnamomum verum and Nigella sativa against Staphylococcus aureus using L-optimal mixture design [Internet]. AMB Express. 2025;15(1). DOI: 10.1186/s13568-024-01797-y

20. Azhdarzadeh F, Hojjati M. Chemical composition and antimicrobial activity of leaf, ripe and unripe peel of bitter orange (Citrus aurantium) essential oils [Internet]. Nutr Food Sci Res. 2016;3(1):43–50. DOI: 10.18869/acadpub.nfsr.3.1.43

21. Romano KP, Hung DT. Targeting LPS biosynthesis and transport in gram-negative bacteria in the era of multi-drug resistance [Internet]. Biochim Biophys Acta Mol Cell Res. 2023;1870(3):119407. DOI: 10.1016/j.bbamcr.2022.119407

22. Winkelman WJ. Aromatherapy, botanicals, and essential oils in acne [Internet]. Clin Dermatol. 2018;36(3):299–305. DOI: 10.1016/j.clindermatol.2018.03.004

23. Munda S, Pandey SK, Dutta S, Baruah J, Lal M. Antioxidant Activity, Antibacterial Activity and Chemical Composition of Essential Oil of Artemisia vulgaris L. Leaves from Northeast India [Internet]. J Essent Oil Bear Plants. 2019;22(2):368–79. DOI: 10.1080/0972060X.2019.1602083

24. Bassolé IH, Lamien-Meda A, Bayala B, Tirogo S, Franz C, Novak J, et al. Composition and antimicrobial activities of Lippia multiflora Moldenke, Mentha x piperita L. and Ocimum basilicum L. essential oils and their major monoterpene alcohols alone and in combination [Internet]. Molecules. 2010;15(11):7825–39. DOI: 10.3390/molecules15117825

25. Soulaimani B. Comprehensive Review of the Combined Antimicrobial Activity of Essential Oil Mixtures and Synergism with Conventional Antimicrobials [Internet]. Nat Prod Commun. 2025;20(3):1–22. DOI: 10.1177/1934578X251328241

26. Moussii IM, Nayme K, Timinouni M, Jamaleddine J, Filali H, Hakkou F. Synergistic antibacterial effects of Moroccan Artemisia herba alba, Lavandula angustifolia and Rosmarinus officinalis essential oils [Internet]. Synergy. 2020; 10(6):100057. DOI:10.1016/j.synres.2019.100057

27. Soulaimani B, El Hidar N, El Fakir SB, Mezrioui N, Hassani L, Abbad A. Combined antibacterial activity of essential oils extracted from Lavandula maroccana (Murb.), Thymus pallidus Batt. and Rosmarinus officinalis L. against antibiotic-resistant Gram-negative bacteria [Internet]. Eur J Integr Med. 2021;43(4):101312. DOI: 10.1016/j.eujim.2021.101312

28. Hossain F, Follett P, Vu KD, Harich M, Salmieri S, Lacroix M. Evidence for synergistic activity of plant-derived essential oils against fungal pathogens of food [Internet]. Food Microbiol. 2016;53:24–30. DOI: 10.1016/j.fm.2015.08.006

29. Basavegowda N, Baek KH. Synergistic Antioxidant and Antibacterial Advantages of Essential Oils for Food Packaging Applications [Internet]. Biomolecules. 2021;11:1267. DOI: 10.3390/biom11091267

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Published

2026-02-20

How to Cite

1.
Tapia Puma VA, Urrunaga Ormachea MJ, del Carpio Jiménez C, Espinoza Carrasco HY, Jordan Quispe KJ, Chavez Vasquez MA, et al. Antibacterial activity of blends of essential oils of Minthostachys setosa and Citrus maxima. Rev. cuba. med. mil [Internet]. 2026 Feb. 20 [cited 2026 Apr. 17];55(1):e026076906. Available from: https://revmedmilitar.sld.cu/index.php/mil/article/view/76906

Issue

Vol. 55 No. 1 (2026): January - March

Section

Research Article

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Copyright (c) 2026 Víctor Andrés Tapia Puma, Mario J. Urrunaga Ormachea, Carla del Carpio Jiménez, Heldy Yiyi Espinoza Carrasco, Karen Jessica Jordan Quispe, Marly Anai Chavez Vasquez , Giancarlo Gutierrez-Chavez

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