Zebrafish as a biomodel in oncology, applications in the evaluation of therapies and gene vectors
Keywords:
biological model; gene therapies; oncology; zebrafishAbstract
Introduction: Zebrafish (Danio rerio) has become a fundamental biological model in experimental medicine and oncology due to its high genetic homology with humans (82% in disease-associated genes), embryonic transparency, and ease of genetic manipulation.
Objective: To review the role of zebrafish in cancer research, focusing on its application for studying tumor progression and its relevance in innovative areas such as gene therapies.
Methods: A review of current scientific literature was conducted, analyzing studies that use zebrafish as a model in oncology. Studies without relevant experimental validation, opinion articles without empirical basis, or research that did not establish correlation with human cancer models were excluded.
Development: The transparency of the model and the development of transgenic lines facilitate direct observation and tracking of tumor cells. In gene therapy, zebrafish has been instrumental in testing and optimizing therapeutic gene delivery systems, both viral and non-viral. Furthermore, its use in preclinical studies has enabled the validation of innovative therapies, such as CAR-T cells, and genome editing strategies with CRISPR/Cas9, accelerating the development of targeted treatments.
Conclusions: Zebrafish constitutes a key vertebrate model for oncology, allowing real-time visualization of tumor progression and interaction with the microenvironment, while its high feasibility for gene editing positions it as a central platform in the development of innovative gene therapies.
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Copyright (c) 2026 Thalía Sánchez Miralles, Sheyla Fernández Puentes, Kamila Morín Fanego, Yenia Aldana Mulet, Dianelys Cabrera Márquez, Anabel Cisneros Campo, Sonia García Lago, Silvia García Viamonte, Luis Arturo Fonseca-Fonseca, Jorge Berlanga Acosta, Ché Serguera, Yanier Nunez-Figueredo
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