Pruebas moleculares para la detección de SARS-CoV-2
Resumen
Objetivo: Describir las pruebas moleculares para la detección de SARS-CoV-2.
Desarrollo: Se han diseñado tres tipos de ensayos de rRT-PCR, para la detección de SARS-CoV-2. Las pruebas descritas son especÃficas para genes ARN (polymerase dependent RNA - RdRp), genes E (upE) y genes Nnes (N). Los Centros para la Prevención y Control de Enfermedades (CDC) de los EE.UU., han desarrollado la rRT-PCR dirigidos a la proteÃna N de la nucleocápside del material genético SARS-CoV-2, los ensayos genes E (upE) y gen de la proteÃna 1b (ORF 1b) se pueden complementar para la detección y confirmación.
Conclusiones: Las dianas que se emplean para la detección especÃfica del genoma del SARS-CoV-2 por RT-PCR en tiempo real, se encuentran en las regiones ORF1a y 1b, RdRp, N, S y E del ARN viral.
Palabras clave
Referencias
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