Optimization of a honey-based culture medium for Escherichia coli

Authors

Keywords:

honey, Apis mellifera, Escherichia coli, bees.

Abstract

Introduction: Optimization of culture media in most cases has been done by empirical procedures. Factorial design and response surface methodology are used to make a medium more productive. Objectives: To optimize the productivity of a solid culture medium based on Apis mellifera honey for Escherichia coli.
Methods: Quantitative, in vitro and comparative approach, a preliminary phytochemical assay of honey was performed. A factorial design (22) was applied where the influence of incubation time and the amount of honey in culture media was evaluated. Forty agar base plates with honey were used, distributed in 4 groups (n = 10): group I (agar base with 10% honey and incubation for 12 hours), group II (agar base with 10% honey and incubation for 24 hours), group III (agar base with 20% honey and incubation for 12 hours) and group IV (agar base with 20% honey and incubation for 24 hours). The ecometric technique was used to determine productivity.
Results: In the phytochemical assay, carbohydrates, alkaloids, triterpenoids and cardiotonic glycosides were detected. The medium productivity of groups II and III was verified. The main and interaction effects were significant and the prediction index of the model was adequate.
Conclusions: The optimized solid culture medium based on 20% bee honey and incubation for 24 hours for Escherichia coli shows high productivity.

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Author Biographies

Héctor Alexander Vílchez Cáceda, Universidad Inca Garcilaso de la Vega

Laboratorio de Investigación y Desarrollo Farmacéutico

Antonella Rosario Olortegui Quispe, UNIVERSIDAD INCA GARCILASO DE LA VEGA

Químico farmacéutico colegiada. En el transcurso de los últimos años de su formación profesional, se ha desempeñado como asistente de laboratorio en las asignaturas de microbiología, bioquímica, química inorgánica y orgánica, farmacognosia y fitoquímica. Realizó sus prácticas pre-profesionales en una Clínica privada en el área de farmacia y laboratorio de preparados magistrales. Como profesional se ha desempeñado como analista de calidad y asistente en asuntos regulatorios de productos farmacéuticos.

Willian Esteban Chu Estrada, UNIVERSIDAD INCA GARCILASO DE LA VEGA

Docente Ordinario en la categoría de Principal de la Universidad Autónoma de Ica, con grado de Doctor en Contabilidad por la Universidad San Pedro de Chimbote, 15 años de docente con experiencia en áreas académicas, contables, administrativas. Investigador Científico registrado en Concytec, Docente en la Escuela de Postgrado de la Universidad Nacional José Faustino Sánchez Carrión.

Christhian Alexander Alvia Saldarriaga, UNIVERSIDAD MARÍA AUXILIADORA

Químico farmacéutico con orientación hacia la investigación. Cuento con experiencia en el rubro de farmacia hospitalaria, lo que incluye actividades como seguimiento farmacoterapéutico, elaboración de reportes de sospechas de eventos adversos y de causalidad. Además en el rubro de la industria farmacéutica cuenta con conocimiento en el proceso de manufactura de medicamentos. Durante mi periodo académico fui asistente de catedra de las asignaturas de microbiología, parasitología y fitoquímica. Por otro lado brinde apoyo en el laboratorio académico para la preparación de clases así como en los procedimientos experimentales para diferentes artículos de investigación llevados a cabo por diferentes docentes universitarios

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Published

2023-07-04

How to Cite

1.
Vílchez Cáceda HA, Olortegui Quispe AR, Chu Estrada WE, Alvia Saldarriaga CA. Optimization of a honey-based culture medium for Escherichia coli. Rev Cubana Med Milit [Internet]. 2023 Jul. 4 [cited 2025 Jan. 30];52(3):e02302821. Available from: https://revmedmilitar.sld.cu/index.php/mil/article/view/2821

Issue

Vol. 52 No. 3 (2023): July - september

Section

Research Article

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