Epidermal growth factor and remarkable correlations in a case series COVID-19
Keywords:
biomarkers, epidermal growth factor, COVID-19.Abstract
Introduction: Molecular links relate epidermal growth factor (EGF) to inflammatory phenomena. In the context of COVID-19, understanding the role of serum concentrations of EGF offered new possibilities for a better understanding of physiopathology and therapeutics.Objective: To explore the behavior of serum EGF values in patients with COVID-19, as well as to determine possible significant correlations between EGF, and analytical and clinical parameters of interest.
Methods: Cross-sectional observational analytical study, in a series of COVID-19 cases that included 15 patients attended between august-september 2021. Variables included: Age, sex, comorbidities, respiratory rate, heart rate, hospital stay, neutrophils, lymphocytes, neutrophil-lymphocyte index. Summary measures: Absolute frequency, percentage, and the arithmetic mean were used. The statistical significance of observable differences between groups was explored with the chi-square test or Welch's t test with a= 0.05.
Results: Notable correlation observed are rEGF-age= -0.6211 (p= 0.0206) overall, with rEGF-age= 0.2998 (p= 0.4030) in critically ill patients and rEGF-age= -0.9607 (p= 0.0000) in patients with care report. In the case of correlation with neutrophils, this is observed in the case of the subset of critical patients (rEGF-PNN= -0.4471, p= 0.3524); in relation to respiratory rate and heart rate, remarkably strong correlations were observed in critically ill patients (rEGF-Rr= 0.8220, p= 0.1928; rEGF-Hr= -0.9285, p= 0.1207).
Conclusions: There is a plausible relationship between serum EGF values and COVID-19, with notably strong correlations for clinical parameters in the context of serious illness.
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References
1. Zhao C, Yang L, Zhou F, Yu Y, Du X, Xiang Y, et al. Feedback activation of EGFR is the main cause for STAT3 inhibition-irresponsiveness in pancreatic cancer cells. Oncogene. 2020; 39(20):3997-4013. DOI: 10.1038/s41388-020-1271-y
2. Dosch AR, Dai X, Reyzer ML, Mehra S, Srinivasan S, Willobee BA, et al. Combined Src/EGFR Inhibition Targets STAT3 Signal-ing and Induces Stromal Remodeling to Improve Survival in Pancreatic Cancer. Mol Cancer Res. 2020; 18(4):623-31. DOI: 10.1158/1541-7786.mcr-19-0741
3. Gadanec LK, McSweeney KR, Qaradakhi T, Ali B, Zulli A, Apostolopoulos V. Can SARS-CoV-2 Virus Use Multiple Receptors to Enter Host Cells. Int J Mol Sci. 2021; 22(3):992. DOI: 10.3390/ijms22030992
4. Yoo J, Rodriguez Perez CE, Nie W, Edwards RA, Sinnett-Smith J, Rozengurt E. TNF-a induces upregulation of EGFR expression and signaling in human colonic myofibroblasts. Am J Physiol Gastrointest Liver Physiol. 2012; 302(8):G805-14. DOI: 10.1152/ajpgi.00522.2011
5. Harrison PT, Vyse S, Huang PH. Rare epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer. Semin Cancer Biol. 2020; 61:167-79. DOI: 10.1016/j.semcancer.2019.09.015
6. Laudanski K, Okeke T, Hajj J, Siddiq K, Rader DJ, Wu J, et al. Longitudinal urinary biomarkers of immunological activation in covid-19 patients without clinically apparent kidney disease versus acute and chronic failure. Sci Rep. 2021; 11(1):19675. DOI: 10.1038/s41598-021-99102-5
7. Tay MZ, Poh CM, Rénia L, MacAry PA, Ng LFP. The trinity of COVID-19: immunity, inflammation and intervention. Nat Rev Imanol. 2020; 20(6):363-74. DOI: 10.1038/s41577-020-0311-8
8. Merad M, Martin JC. Pathological inflammation in patients with COVID-19: a key role for monocytes and macrophages. Nature Reviews Immunology. 2020; 20(6):355-62. DOI: 10.1038/s41577-020-0353-y
9. Cuba. Ministerio de Salud Pública. Protocolo de Actuación Nacional para la Covid-19. Versión 1.7. La Habana: Minsap; 2021.
10. Ministerio de Salud Pública. Regulación D 03-21 Buenas Prácticas de Laboratorio Clínico. Centro para el Control Estatal de Medicamentos Equipos y Dispositivos médicos (CECMED). 2021. [acceso: 22/04/2021]. Disponible en: https://www.cecmed.cu/sites/default/files/adjuntos/Reglamentacion/ResRegBPLC%20-firmada.pdf
11. Borda Pérez M. Métodos Cuantitativos. Herramientas para la investigación en salud. 4a ed. Baranquilla: Universidad del Norte; 2014. 360 p.
12. World Medical Association. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2013. 310(20):2191-4 DOI: 10.1001/jama.2013.281053
13. Ministerio de Salud Pública. Regulación 165-2000: Buenas Prácticas clínicas en Cuba. Centro para el Control Estatal de Medicamentos Equipos y Dispositivos médicos (CECMED). 2016. [acceso: 22/04/2021]. Disponible en: https://www.cecmed.cu/sites/default/files/adjuntos/Reglamentacion/regulacion_requi-sitos_registro_sanitario_biologicosmod.pdf
14. International Council for Harmonisation (ICH). ICH-E6 Good Clinical Practice (GCP). ICH; 2021. [acceso: 22/04/2021]. Disponible en: https://database.ich.org/sites/default/files/ICH_E6-R3_GCP-Principles_Draft_2021_0419.pdf
15. Abdelmageed MM, Kefaloyianni E, Arthanarisami A, Komaru Y, Atkinson JJ, Herrlich A. TNF or EGFR inhibition equally block AKI-to-CKD transition: opportunities for etanercept treatment. Nephrol Dial Transplant. 2023; 38(5):1139-50. DOI: 10.1093/ndt/gfac290
16. Idasiak-Piechocka I, Miedziaszczyk M, Wozniak A, Pawliczak E, Kaczmarek E, Oko A. Interleukin-6 and epidermal growth factor as noninvasive biomarkers of progression in chronic glomerulonephritis. Am J Physiol Cell Physiol. 2023; 325(5):1267-75. DOI: 10.1152/ajpcell.00058.2023
17. Schumacher N, Rose-John S. ADAM17 orchestrates Interleukin-6, TNFa and EGF-R signaling in inflammation and cancer. Biochim Biophys Acta Mol Cell Res. 2022; 1869(1):119141. DOI: 10.1016/j.bbamcr.2021.119141
18. Mendoza-Marí Y, García-Ojalvo A, Fernández-Mayola M, Rodríguez-Rodríguez N, Martinez-Jimenez I, Berlanga-Acosta J. Epidermal growth factor effect on lipopolysaccharide-induced inflammation in fibroblasts derived from diabetic foot ulcer. Scars Burn Heal. 2022; 8:1-11. DOI: 10.1177/20595131211067380
19. Idania G, Hassiul, CL, Adriana CP, Kalet LM. Measurement of Serum EGF Levels, a Methodological Approach: Learning What Means Low-/High-Concentration of EGF In Serum. Some Clinical Implications. J Mol Biomark Diagn. 2017; 8(3):1000335. DOI: 10.4172/2155-9929.1000335
20. Mathilde IK, Aalund OD, Alnor A, Brandslund I, Bechmann T, Madsen JK. EGFR and EGFR ligands in serum in healthy women; reference intervals and age dependency. Clin Chem Lab Med. 2019; 57(12):1948-55. DOI: 10.1515/cclm-2019-0376
21. Joh T, Itoh M, Katsumi K, Yokoyama Y, Takeuchi T, Kato T, et al. Physiological concentrations of human epidermal growth factor in biological fluids: use of a sensitive enzyme immunoassay. Clin Chim Acta. 1986; 158(1):81-90. DOI: 10.1016/0009-8981(86)90118-x
2. Dosch AR, Dai X, Reyzer ML, Mehra S, Srinivasan S, Willobee BA, et al. Combined Src/EGFR Inhibition Targets STAT3 Signal-ing and Induces Stromal Remodeling to Improve Survival in Pancreatic Cancer. Mol Cancer Res. 2020; 18(4):623-31. DOI: 10.1158/1541-7786.mcr-19-0741
3. Gadanec LK, McSweeney KR, Qaradakhi T, Ali B, Zulli A, Apostolopoulos V. Can SARS-CoV-2 Virus Use Multiple Receptors to Enter Host Cells. Int J Mol Sci. 2021; 22(3):992. DOI: 10.3390/ijms22030992
4. Yoo J, Rodriguez Perez CE, Nie W, Edwards RA, Sinnett-Smith J, Rozengurt E. TNF-a induces upregulation of EGFR expression and signaling in human colonic myofibroblasts. Am J Physiol Gastrointest Liver Physiol. 2012; 302(8):G805-14. DOI: 10.1152/ajpgi.00522.2011
5. Harrison PT, Vyse S, Huang PH. Rare epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer. Semin Cancer Biol. 2020; 61:167-79. DOI: 10.1016/j.semcancer.2019.09.015
6. Laudanski K, Okeke T, Hajj J, Siddiq K, Rader DJ, Wu J, et al. Longitudinal urinary biomarkers of immunological activation in covid-19 patients without clinically apparent kidney disease versus acute and chronic failure. Sci Rep. 2021; 11(1):19675. DOI: 10.1038/s41598-021-99102-5
7. Tay MZ, Poh CM, Rénia L, MacAry PA, Ng LFP. The trinity of COVID-19: immunity, inflammation and intervention. Nat Rev Imanol. 2020; 20(6):363-74. DOI: 10.1038/s41577-020-0311-8
8. Merad M, Martin JC. Pathological inflammation in patients with COVID-19: a key role for monocytes and macrophages. Nature Reviews Immunology. 2020; 20(6):355-62. DOI: 10.1038/s41577-020-0353-y
9. Cuba. Ministerio de Salud Pública. Protocolo de Actuación Nacional para la Covid-19. Versión 1.7. La Habana: Minsap; 2021.
10. Ministerio de Salud Pública. Regulación D 03-21 Buenas Prácticas de Laboratorio Clínico. Centro para el Control Estatal de Medicamentos Equipos y Dispositivos médicos (CECMED). 2021. [acceso: 22/04/2021]. Disponible en: https://www.cecmed.cu/sites/default/files/adjuntos/Reglamentacion/ResRegBPLC%20-firmada.pdf
11. Borda Pérez M. Métodos Cuantitativos. Herramientas para la investigación en salud. 4a ed. Baranquilla: Universidad del Norte; 2014. 360 p.
12. World Medical Association. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2013. 310(20):2191-4 DOI: 10.1001/jama.2013.281053
13. Ministerio de Salud Pública. Regulación 165-2000: Buenas Prácticas clínicas en Cuba. Centro para el Control Estatal de Medicamentos Equipos y Dispositivos médicos (CECMED). 2016. [acceso: 22/04/2021]. Disponible en: https://www.cecmed.cu/sites/default/files/adjuntos/Reglamentacion/regulacion_requi-sitos_registro_sanitario_biologicosmod.pdf
14. International Council for Harmonisation (ICH). ICH-E6 Good Clinical Practice (GCP). ICH; 2021. [acceso: 22/04/2021]. Disponible en: https://database.ich.org/sites/default/files/ICH_E6-R3_GCP-Principles_Draft_2021_0419.pdf
15. Abdelmageed MM, Kefaloyianni E, Arthanarisami A, Komaru Y, Atkinson JJ, Herrlich A. TNF or EGFR inhibition equally block AKI-to-CKD transition: opportunities for etanercept treatment. Nephrol Dial Transplant. 2023; 38(5):1139-50. DOI: 10.1093/ndt/gfac290
16. Idasiak-Piechocka I, Miedziaszczyk M, Wozniak A, Pawliczak E, Kaczmarek E, Oko A. Interleukin-6 and epidermal growth factor as noninvasive biomarkers of progression in chronic glomerulonephritis. Am J Physiol Cell Physiol. 2023; 325(5):1267-75. DOI: 10.1152/ajpcell.00058.2023
17. Schumacher N, Rose-John S. ADAM17 orchestrates Interleukin-6, TNFa and EGF-R signaling in inflammation and cancer. Biochim Biophys Acta Mol Cell Res. 2022; 1869(1):119141. DOI: 10.1016/j.bbamcr.2021.119141
18. Mendoza-Marí Y, García-Ojalvo A, Fernández-Mayola M, Rodríguez-Rodríguez N, Martinez-Jimenez I, Berlanga-Acosta J. Epidermal growth factor effect on lipopolysaccharide-induced inflammation in fibroblasts derived from diabetic foot ulcer. Scars Burn Heal. 2022; 8:1-11. DOI: 10.1177/20595131211067380
19. Idania G, Hassiul, CL, Adriana CP, Kalet LM. Measurement of Serum EGF Levels, a Methodological Approach: Learning What Means Low-/High-Concentration of EGF In Serum. Some Clinical Implications. J Mol Biomark Diagn. 2017; 8(3):1000335. DOI: 10.4172/2155-9929.1000335
20. Mathilde IK, Aalund OD, Alnor A, Brandslund I, Bechmann T, Madsen JK. EGFR and EGFR ligands in serum in healthy women; reference intervals and age dependency. Clin Chem Lab Med. 2019; 57(12):1948-55. DOI: 10.1515/cclm-2019-0376
21. Joh T, Itoh M, Katsumi K, Yokoyama Y, Takeuchi T, Kato T, et al. Physiological concentrations of human epidermal growth factor in biological fluids: use of a sensitive enzyme immunoassay. Clin Chim Acta. 1986; 158(1):81-90. DOI: 10.1016/0009-8981(86)90118-x
Published
2024-06-28
How to Cite
1.
Pérez Hernández HJ. Epidermal growth factor and remarkable correlations in a case series COVID-19. Rev Cubana Med Milit [Internet]. 2024 Jun. 28 [cited 2025 Apr. 5];53(3):e024028646. Available from: https://revmedmilitar.sld.cu/index.php/mil/article/view/28646
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