Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolates
Keywords:
beta-Lactamases, cephalosporin resistance, enterobacteriaceae, genetic epidemiology.Abstract
Introduction: Plasmid-mediated class C ß-lactamase (pAmpC) is a member of broad-spectrum ß-lactamase that spreads worldwide. However, its prevalence was under-evaluated.
Objective: To characterize the prevalence and distribution of pAmpC types in 294 cefoxitin (FOX) and third-generation cephalosporin (3GC) resistant Enterobacteriaceae collected in several regions of Thailand and Vietnam in 2018 and 2020.
Methods: Multiplex Polymerase Chain Reaction (PCR) for pAmpC identification was utilized to investigate prevalence and diversification of pAmpC among 294 cefoxitin and third-generation cephalosporin resistant Enterobacteriaceae isolated from Thailand (n= 197) and Vietnam (n= 97).
Results: The prevalence of pAmpC was 37.1 % found in second and third-generation cephalosporin resistant Enterobacteriaceae. CMY-2 like was prominent in Thailand and Vietnam; however, prevalence of CMY-2 was varied in each hospital. DHA contributed 25.7 %, ACT/MIR rate was dominant in Chiang Rai hospital, reached 100 % in Thanh Hoa Pediatrics hospital. Worrisome, 3.7 % - isolates carried two types of pAmpC. The incidence of pAmpC in Vietnam was significantly higher than those in Thailand.
Conclusions: These findings provide evidence-based of highly spreading and diversified distribution of transferable AmpC among Enterobacteriaceae in two Asia-Pacific countries.
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2. Jacoby GA. AmpC beta-lactamases [Internet]. Clinical microbiology reviews. 2009;22(1):161-82. DOI:10.1128/CMR.00036-08
3. Tamma PD, Doi Y, Bonomo RA, Johnson JK, Simner PJ. A Primer on AmpC ß-Lactamases: Necessary Knowledge for an Increasingly Multidrug-resistant World [Internet]. Clinical infectious diseases. 2019;69(8):1446-55. DOI:10.1093/cid/ciz173
4. Ibrahim ME, Abbas M, Al-Shahrai AM, Elamin BK. Phenotypic Characterization and Antibiotic Resistance Patterns of Extended-Spectrum ß-Lactamase- and AmpC ß-Lactamase-Producing Gram-Negative Bacteria in a Referral Hospital, Saudi Arabia [Internet]. The Canadian journal of infectious diseases & medical microbiology. 2019;2019:6054694. DOI:10.1155/2019/6054694
5. Shahid M, Sobia F, Singh A, Khan HM, Hawkey PM, Huq A, et al. AmpC ß-lactamases and bacterial resistance: an updated mini review [Internet]. Rev Med Microbiol. 2009;20(3):41-55. DOI:10.1097/MRM.0b013e328331ad83
6. Zhou Q, Tang M, Zhang X, Lu J, Tang X, Gao Y. Detection of AmpC ß-lactamases in gram-negative bacteria [Internet]. Heliyon. 2022;8(12):e12245. DOI:10.1016/j.heliyon.2022.e12245
7. Philippon A, Arlet G, Labia R, Iorga BI. Class C ß-Lactamases: Molecular Characteristics [Internet]. Clinical microbiology reviews. 2022;35(3):e0015021. DOI:10.1128/cmr.00150-21
8. Tenover FC, Emery SL, Spiegel CA, Bradford PA, Eells S, Endimiani A, et al. Identification of plasmid-mediated AmpC beta-lactamases in Escherichia coli, Klebsiella spp., and Proteus species can potentially improve reporting of cephalosporin susceptibility testing results [Internet]. Journal of clinical microbiology. 2009;47(2):294-9. DOI:10.1128/JCM.01797-08
9. Ingram PR, Inglis TJ, Vanzetti TR, Henderson BA, Harnett GB, Murray RJ. Comparison of methods for AmpC beta-lactamase detection in Enterobacteriaceae [Internet]. Journal of medical microbiology. 2011;60(Pt 6):715-21. DOI:10.1099/jmm.0.029140-0
10. Sakanashi D, Miyazaki N, Kawamoto Y, Ohno T, Yamada A, Koita I, et al. A novel disk-based detection method with superior sensitivity for ß-lactamase production in third-generation cephalosporin-resistant Enterobacteriaceae [Internet]. Journal of infection and chemotherapy. 2019;25(5):330-6. DOI:10.1016/j.jiac.2018.12.008
11. Wagner K, Mancini S, Ritter C, Böttger EC, Keller PM. Evaluation of the AID AmpC line probe assay for molecular detection of AmpC-producing Enterobacterales [Internet]. Journal of global antimicrobial resistance. 2019;19:8-13. DOI:10.1016/j.jgar.2019.04.015
12. CLSI. Ferformance standards for antimicrobial susceptibility testing [Internet]. 30th ed. Wayne, PA: Clinical and Laboratory Standards Institute; 2020. [access: 15/09/2023]. Available at: https://www.nih.org.pk/wp-content/uploads/2021/02/CLSI-2020.pdf
13. Chuong LV, Prachayasittikul V, Isarankura Na Ayudhya C, Lawung R. Multiplex PCR scheme for variant plasmid mediated class C beta-lactamase typing [Internet]. Journal of clinical laboratory analysis. 2018 Mar;32(3). DOI:10.1002/jcla.22298
14. Harris PN, Ferguson JK. Antibiotic therapy for inducible AmpC beta-lactamase-producing Gram-negative bacilli: what are the alternatives to carbapenems, quinolones and aminoglycosides? [Internet]. International journal of antimicrobial agents. 2012;40(4):297-305. DOI:10.1016/j.ijantimicag.2012.06.004
15. Perera P, Gamage S, De Silva HSM, Jayatilleke SK, de Silva N, Aydin A, et al. Phenotypic and genotypic distribution of ESBL, AmpC ß-lactamase and carbapenemase-producing Enterobacteriaceae in community-acquired and hospital-acquired urinary tract infections in Sri Lanka [Internet]. Journal of global antimicrobial resistance. 2022;30:115-22. DOI:10.1016/j.jgar.2022.05.024
16. Gajamer VR, Bhattacharjee A, Paul D, Ingti B, Sarkar A, Kapil J, et al. High prevalence of carbapenemase, AmpC ß-lactamase and aminoglycoside resistance genes in extended-spectrum ß-lactamase-positive uropathogens from Northern India [Internet]. Journal of global antimicrobial resistance. 2020;20:197-203. DOI:10.1016/j.jgar.2019.07.029
17. Rizi KS, Mosavat A, Youssefi M, Jamehdar SA, Ghazvini K, Safdari H, et al. High prevalence of bla(CMY) AmpC beta-lactamase in ESBL co-producing Escherichia coli and Klebsiella spp. clinical isolates in the northeast of Iran [Internet]. Journal of global antimicrobial resistance. 2020;22:477-82. DOI:10.1016/j.jgar.2020.03.011
18. Donà V, Scheidegger M, Pires J, Furrer H, Atkinson A, Babouee Flury B. Gradual in vitro Evolution of Cefepime Resistance in an ST131 Escherichia coli Strain Expressing a Plasmid-Encoded CMY-2 ß-Lactamase [Internet]. Frontiers in microbiology. 2019;10:1311. DOI:10.3389/fmicb.2019.01311
Published
2024-05-17
How to Cite
1.
Le CV, Tran NN, Nguyen PTB, Le NT, Le DT. Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolates. Rev Cubana Med Milit [Internet]. 2024 May 17 [cited 2025 Apr. 2];53(2):e024038119. Available from: https://revmedmilitar.sld.cu/index.php/mil/article/view/38119
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