Antibacterial effect of the hydroalcoholic extract of Tillandsia maculata (Bromeliaceae) on Streptococcus mutans

Authors

Keywords:

anti-bacterial agents, dental caries, chlorhexidine, Tillandsia, Streptococcus mutans.

Abstract

Introduction: Tillandsia maculata Ruíz & Pav. It has secondary metabolites that can affect the development of Streptococcus mutans, the main agent that causes dental caries.
Objectives: To evaluate the antibacterial effect of the hydroalcoholic extract of Tillandsia maculata Ruíz & Pav. on Streptococcus mutans.
Methods: Experimental, in vitro and comparative work. An initial phytochemical screening of the extract was carried out. 56 Müller-Hinton agar plates (Merck®) were used, divided into 7 groups (n= 8): group I (deionized water), group II (70% ethanol), group III (0.12% chlorhexidine), group IV (Tillandsia maculata at 25%), group V (Tillandsia maculata at 50%), group VI (Tillandsia maculata at 75%) and group VII (Tillandsia maculata at 100%). The disk diffusion method detailed by Bauer and Kirby was used; The bacteria used was Streptococcus mutans ATCC 25175 and the evaluations of the inhibition diameters were made after 24 hours, to indicate antibacterial impact.
Results: Phytochemical screening revealed tannins, flavonoids, cardiotonic glycosides and lactones. The antibacterial effect of group VII (Tillandsia maculata at 100 %) was verified with 19.691 ± 0.0679 mm (99.06%), comparable with chlorhexidine at 0.12% (group III) 19.878 ± 0.0451 mm (100%) on Streptococcus mutans.
Conclusions: The hydroalcoholic extract of Tillandsia maculata Ruíz & Pav. 100% exhibits in vitro antibacterial effect on Streptococcus mutans ATCC 25175 with measurements similar to 0.12% chlorhexidine.

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Author Biographies

Héctor Alexander Vílchez-Cáceda, Universidad Inca Garcilaso de la Vega

Laboratorio de Microbiología

Ketty Rojas-Berastein, Universidad Inca Garcilaso de la Vega

Laboratorio de Microbiología

Carolina Mayo Takahashi-Ferrer, Universidad Norbert Wiener

Laboratorio de Fitoquímica

Christhian Alexander Alvia-Saldarriaga, Universidad Norbert Wiener

Laboratorio de Fitoquímica

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Published

2024-05-17

How to Cite

1.
Vílchez-Cáceda HA, Rojas-Berastein K, Takahashi-Ferrer CM, Alvia-Saldarriaga CA. Antibacterial effect of the hydroalcoholic extract of Tillandsia maculata (Bromeliaceae) on Streptococcus mutans. Rev Cubana Med Milit [Internet]. 2024 May 17 [cited 2025 Jun. 12];53(2):e024038860. Available from: https://revmedmilitar.sld.cu/index.php/mil/article/view/38860

Issue

Vol. 53 No. 2 (2024): April - June

Section

Research Article

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