Evaluation of direct microscopic examination and its comparison with sedimentation method for the detection enteroparasites
Keywords:
Blastocystis hominis, parasites, Physical Exercise, Human Physical Fitness, Occupational Health, Public Health, High-Intensity Interval Training, sedimentation, sensitivity, sensitivity and specificity.Abstract
Introduction: Enteroparasites constitute a considerable challenge to public health, particularly in developing nations. These infections, which can cause serious problems in vulnerable groups, require accurate diagnoses. This study analyzes and contrasts diagnostic techniques that evaluate sensitivity and specificity, with the goal of discovering effective and affordable options in various contexts.
Objective: To evaluate direct microscopic examination and compare with the sedimentation method for the detection of enteroparasites.
Methods: A quantitative, descriptive cross-sectional design was carried out; 50 fecal samples were analyzed using direct microscopic examination and the sedimentation method. Data were analyzed in SPSS to determine the sensitivity and specificity of both methods, using the sedimentation method as a reference.
Results: Direct microscopic examination detected parasites in 64,0% of the samples, while the sedimentation method identified 72,0%. Blastocystis hominis was the most frequent parasite in both techniques. The sensitivity of direct microscopic examination was 80,56% and its specificity was 78,57%.
Conclusions: The sedimentation method presents greater sensitivity and specificity compared to direct microscopic examination, although the latter remains useful in contexts with limited resources. The choice of diagnostic technique should be based on the available resources and the needs of the clinical environment.
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1.Catrileo Daniela L, Zulantay Alfaro I, Apt Baruch W, Canals Lambarri M. Mortalidad por parasitosis endémicas e importadas en Chile [Internet]. Rev chil infectol. 2022 [acceso: 13/07/2024]; 39(2): 138-48. Disponible en: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182022000200138&lng=es
2.Villca Chuquichambi M, Vargas Madrid D. Prevalencia de parásitos intestinales en pacientes que acudieron al laboratorio Laboclin S.R.L [Internet]. Revista UNITEPC. 2023 [acceso: 10/08/2024]; 10(2): 36-43. Disponible en: http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S2520-98252023000200036&lng=es
3. Aldea Gonzales C, Villca Chuquichambi M, Ontiveros Oporto J, Vargas Mamani S, Rubén Darío Ríos Cruz. Frecuencia de la enteroparasitosis y factores de riesgo asociados en manipuladores de alimentos en el Municipio de Vinto, Cochabamba, Bolivia [Internet]. Revista De Investigación E Información En Salud. 2024; 9(47): 50-58. DOI: https://doi.org/10.52428/20756208.v19i47.1182
4. Ayele A, Tegegne Y, Derso A, Eshetu T, Zeleke AJ. Prevalence and associated factors of intestinal helminths among kindergarten children in Gondar town, northwest Ethiopia [Internet]. Pediatric Health Med Ther. 2021 [acceso: 13/07/2024]; 12:35-41. Disponible en: https://www.dovepress.com/prevalence-and-associated-factors-of-intestinal-helminths-among-kinder-peer-reviewed-fulltext-article-PHMT
5. Villacreses LL, Gonzalez Vera L. Vista de Aplicación, cálculo e importancia de la sensibilidad, especificidad y valor predictivo de las pruebas de diagnóstico en el laboratorio clínico [Internet]. Ciencias de la Salud. 2021 [acceso:13/07/2024]; 10: e23857. Disponible en: https://dialnet.unirioja.es/descarga/articulo/8229763.pdf
6. Carrasco Solano F, Santa Cruz López C, Vergara Espinoza M, Sánchez Fernandez M. Comparación de técnicas coproparasitológicas para el diagnóstico de geohelmintos intestinales en niños Lambayecanos [Internet]. Gac Med Bol. 2023 [acceso: 13/07/2024]; 46(1): 72-6. Disponible en: http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S1012-29662023000100072&lng=es
7. Demeke G, Fenta A, Dilnessa T. Evaluation of wet mount and concentration techniques of stool examination for intestinal parasites identification at Debre Markos comprehensive specialized hospital, Ethiopia [Internet]. Infect Drug Resist. 2021 [acceso: 13/07/2024];14: 1357-62. Disponible en: https://www.dovepress.com/evaluation-of-wet-mount-and-concentration-techniques-of-stool-examinat-peer-reviewed-fulltext-article-IDR
8. Alonso Rosales R, Bautista Manchego K. Comparación de tres métodos de concentración de enteroparásitos en muestras fecales humanas [Internet]. Rev Cubana Med Trop. 2020 [acceso: 13/07/2024 ]; 72(2): 2-4. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07602020000200008&lng=es
9. Gonzales Espino KI. Técnica de sedimentación simple versus microtécnica de sedimentación en la identificación de parasitosis intestinales en pacientes del centro de salud del distrito de Pueblo Nuevo, Ica 2018 [Internet]. [Tesis de pregrado]. Perú: Universidad de Alas Peruanas; 2019. [acceso: 27/07/2024]. Disponible en: https://repositorio.uap.edu.pe/bitstream/handle/20.500.12990/8907/Tesis_T%C3%A9cnica_Sedimentaci%C3%B3n_Simple.pdf?sequence=1&isAllowed=y
10. Cortes Reyes E, Rubio Romero J, Gaitan Duarte H. Vista de Métodos estadísticos de evaluación de la concordancia y la reproducibilidad de pruebas diagnósticas. (s/f) [Internet]. Rev Colomb de obstetricia y ginecología. 2010 [acceso: 22/10/2024]; 61(4):e18597. Disponible en: https://revista.fecolsog.org/index.php/rcog/article/view/271/276
11. Terashima A, Marcos L, Maco V, Canales M, Samalvides F, Tello R, et al. Técnica de sedimentación en tubo de alta sensibilidad para el diagnóstico de parásitos intestinales [Internet]. Rev Gastroenterol Perú. 2009 [acceso: 05/05/2024]; 29(4):305-10. Disponible en: http://www.scielo.org.pe/scielo.php?script=sci_arttext&pid=S1022-51292009000400002
12. Jaime Alonso R, Bautista K. Comparación de tres métodos de concentración de enteroparásitos en muestras fecales humanas [Internet]. Revista Cubana de Medicina tropical. 2020 [acceso: 05/05/2024]; 72 (2): 497. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S0375-07602020000200008
13. Salvador F, Goterris L, Sulleiro E, López I, Balladares M, et al. Microscopic examination after concentration techniques for Blastocystis sp. detection in serial faecal samples: How many samples are needed? [Internet]. Parasite Epidemiol Control. 2020 [acceso: 06/09/2024]; 9:e00137. Disponible en: https://pubmed.ncbi.nlm.nih.gov/32055721/
14. Quispe S, Cesar J. Técnica de sedimentación espontánea en tubo para diagnóstico de enteroparasitosis en centros de salud de primer nivel [Internet]. Rev médica - Col Méd Paz. 2017 [acceso: 26/08/2024]; 23(2):13-9. Disponible en: http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S1726-89582017000200003
15. Utzinger J, Becker SL, van Lieshout L, van Dam GJ, Knopp S. New diagnostic tools in schistosomiasis [Internet]. Clin Microbiol Infect. 2015 [acceso: 06/09/2024]; 21(6):529-42. Disponible en: https://pubmed.ncbi.nlm.nih.gov/25843503/
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Copyright (c) 2025 Cristina Aldea Gonzales, Moria Villca Chuquichambi, Miguel Angel Condori Sarzuri, Aranza Rojas Quispe, Camila Florencia Lopez Villegas

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