In vitro antibacterial activity of oral OLEOZON® on bacteria isolated from root canals

Authors

Keywords:

anti-bacterial agents; microbial sensitivity tests; mouth diseases; ozone therapy; plant oils

Abstract

Introduction: Oral OLEOZON® is a natural medicine product effective for intestinal giardiasis and as a therapeutic alternative for various oral conditions; however, its antibacterial properties are not sufficiently documented.

Objective: To evaluate the in vitro antibacterial activity of oral OLEOZON® on bacteria isolated from root canals.

Methods: An in vitro experimental study was carried out. Six bacteria belonging to the Staphylococcus, Enterococcus, Klebsiella, Pseudomonas, and Escherichia kingdoms were analyzed. These bacteria were present in the root canals of patients diagnosed with pulp necrosis and without prior endodontic treatment. Ten replicates were performed per bacterium and per group (total: 240 tests). Antibacterial activity was determined using the agar diffusion method. Inhibition zones, bacterial sensitivity, and the relative inhibitory percentage compared to positive controls were determined. Descriptive and inferential statistics were used.

Results: Oral OLEOZON® showed superior halos against coagulase-negative Staphylococcus (28.7 ± 2.88 mm), Staphylococcus aureus (20.5 ± 2.48 mm), and Escherichia coli (17.2 ± 1.16 mm), with statistically significant differences confirmed by post hoc analysis (p = 0.000). Regarding bacterial susceptibility, isolates treated with oral OLEOZON® were in the very sensitive (41.7%) and extremely sensitive (36.7%) categories. The inhibitory capacity of oral OLEOZON® was 573.0% against erythromycin in cases of Escherichia coli, 410.0% versus azithromycin in cases of Staphylococcus aureus, and 317.2% versus amoxicillin against Klebsiella pneumoneae.

Conclusions: Oral OLEOZON® demonstrated significant antibacterial activity in vitro compared to the antibiotics tested.

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References

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Published

2026-03-31

How to Cite

1.
Alvarez Hernández JC, García Sánchez OM, Pérez García LM, Díaz Luis J, Morales Cabrera M, García Novoa S. In vitro antibacterial activity of oral OLEOZON® on bacteria isolated from root canals. Rev. cuba. med. mil [Internet]. 2026 Mar. 31 [cited 2026 Aug. 9];55(2):e026077137. Available from: https://revmedmilitar.sld.cu/index.php/mil/article/view/77137

Issue

Vol. 55 No. 2 (2026): April - June

Section

Research Article

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Copyright (c) 2026 José Carlos Alvarez Hernández, Orietta Margarita García Sánchez, Lizandro Michel Pérez García, Jacqueline Díaz Luis, Marileidi Morales Cabrera, Sahily García Novoa

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