Genotypic characterization with spacer oligonucleotides of multidrug-resistant isolates of Mycobacterium tuberculosis in Cuba
Keywords:
Mycobacterium tuberculosis complex, spolygotyping, tuberculosis, surveillance, molecular epidemiology.Abstract
Introduction: Mycobacterium tuberculosis with drug resistance has become one of the main obstacles to the control of this disease worldwide and its surveillance is a priority.Objectives: Carry out the molecular characterization of isolates of multidrug-resistant Mycobacterium tuberculosis in Cuba.
Methods: Observational, descriptive, longitudinal and retrospective study, in Cuba, in the period 1995-2014. The sample consisted of 54 isolates. Typing with spacer oligonucleotides was performed. Epidemiological data were obtained from the national anti-tuberculosis drug resistance surveillance survey.
Results: A predominance of the Beijing genotype was observed, followed by the LAM9 sublineage; 83.33 % of the total cases were found in the age range of 15 to 64 years. In the Western and Eastern 81.48 % of the cases are concentrated, however the Western presents more cases and greater genotypic diversity; 75 % of multidrug-resistant cases are new cases. The two factors that favor the appearance of the most frequent multidrug-resistant cases are chronic cases and those from abroad.
Conclusions: This is the first 20-year study of multidrug-resistant isolates in Cuba, where an association between the Beijing lineage and resistance is found. It occurs in patients of working age, and the relationship of the Cuban cases with those reported in the international database makes it essential to monitor the entry into Cuba of multidrug-resistant isolated.
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References
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19. Chung-Delgado K, Guillen-Bravo S, Revilla-Montag A, Bernabe-Ortiz A. Mortality among MDR-TB Cases: Comparison with Drug-Susceptible Tuberculosis and Associated Factors. Plos One. 2015; 10(3): e0119332. DOI: 10.1371/journal.pone.0119332
20. Battista Miglioria G, Tiberi S, Zumlad A, Petersene E, Muhwa Chakayah J, Wejsej C, et al. MDR/XDR-TB management of patients and contacts: Challenges facing the new decade. The 2020 clinical update by the Global Tuberculosis Network. International Journal of Infectious Diseases. 2020; 92S:S15-S25. DOI: 10.1016/j.ijid.2020.01.042
21. Soto Cabezas MG, Munayco Escate CV, Escalante Maldonado O, Valencia Torres E, Arica Gutiérrez J, Yagui Moscoso MJA. Perfil epidemiológico de la tuberculosis extensivamente resistente en el Perú, 2013-2015. Rev Panam Salud Publica. 2020; 44: e29. DOI: 10.26633/RPSP.2020.29
22. Lönnroth K, Mor Z, Erkens C, Bruchfeld R, Nathavitharana R, van der Werf MJ, et al. Tuberculosis in migrants in low-incidence countries: epidemiology and intervention entry points. Int J Tuberc Lung Dis. 2017; 21(6):624–36. DOI: 10.5588/ijtld.16.0845
2. Caminero Luna JA. Retos y posibilidades ante la tuberculosis multirresistente. Arch Bronconeumol. 2017 [acceso: 05/02/2022]; 53(8):417–418. Disponible en: https://www.archbronconeumol.org/index.php?p=revista&tipo=pdf-simple&pii=S0300289617300455
3. Ministerio de Salud Pública. Programa Nacional de Control de la Tuberculosis en Cuba. La Habana: Misnap. [acceso: 29/11/2019]. Disponible en: http://www.sld.cu/galerias/pdf/sitios/tuberculosis/programa_2015.pdf
4. Lemus Molina D, Echemendía Font M, Díaz Rodríguez R, Rodríguez Estévez D, Martínez Rodríguez A, Suárez Álvarez L, et al. Resistencia a fármacos antituberculosos en Cuba, 2015-2017. Revista Cubana de Medicina Tropical. 2021 [acceso: 05/02/2022]; 73(1):e590. Disponible en: http://scielo.sld.cu/pdf/mtr/v73n1/1561-3054-mtr-73-01-e590.pdf
5. Kent PT, Kubica GP. Mycobacteriology: A guide for the level III laboratory. Atlanta: Ga. US Dept of Health and Human Services, Public Health Service, Centers for Disease Control; 1985.
6. Marzouk M, Kahla IB, Hannachi N, Ferjeni A, Salma W, Ghezal S, et al. Evaluation of an immunochromatographic assay for rapid identification of Mycobacterium tuberculosis complex in clinical isolates. Diagn Microbiol Infect Dis. 2011 [acceso: 05/02/2022]; 69(4):396-9. Disponible en: https://pubmed.ncbi.nlm.nih.gov/21396535/
7. van Soolingen D, de Haas P, Kremer K. Restriction fragment length polymorphism (RFLP) typing of mycobacteria. Methods Mol Med. 2001; 54:165-203. DOI: 10.1385/1-59259-147-7:165
8. Kamerbeek J, Schouls L, Kolk A, van Agterveld M, van Soolingen D, Kuijper S, et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 1997 [acceso: 05/02/2022]; 35(4):907-14. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC229700/
9. Couvin D, David A, Zozio T, Rastogi N. Macro-geographical specificities of the prevailing tuberculosis epidemic as seen through SITVIT2, an updated version of the Mycobacterium tuberculosis genotyping database. Infection, Genetics and Evolution. 2019; 72:31–43. DOI: 10.1016/j.meegid.2018.12.030
10. Hunter PR, Gaston MA. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol. 1988 [acceso: 05/02/2022]; 26(11): 2465-66. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC266921/
11. Small PM, Hopewell PC, Singh SP, Paz A, Parsonnet J, Ruston DC, et al. The epidemiology of tuberculosis in San Francisco. A population-based study using conventional and molecular methods. N Engl J Med. 1994; 330(24):1703-9. DOI: 10.1056/NEJM199406163302402
12. Al-Mutairi NM, Ahmad S, Mokaddas EM. Molecular characterization of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) isolates identifies local transmission of infection in Kuwait, a country with a low incidence of TB and MDR-TB. Eur J Med Res. 2019; 24:38. DOI: 10.1186/s40001-019-0397-2
13. Montserrat Alvarez-Corrales N, Pineda-García L, Carrasco Cáceres JA, Aguilar Molina PY. Evaluación de spoligotyping a partir de baciloscopías como metodología alterna e independiente de cultivo para la genotipificación de Mycobacterium tuberculosis. Rev Chilena Infectol. 2021 [acceso: 05/02/2022]; 38(1): 61-8. Disponible en: https://pubmed.ncbi.nlm.nih.gov/33844794/
14. Herrera Avila YM, Fonseca Gómez CM, Gozá Valdés R, Martínez Rodríguez IM, Lemus Molina D, Llanes Cordero MJ, et al. Tipificación con oligonucleótidos espaciadores de Mycobacterium tuberculosis en Cuba. Rev Cubana Med Trop. 2015 [acceso: 21/02/2022]; 67:85-96. Disponible en: http://scielo.sld.cu/pdf/mtr/v67n1/mtr09115.pdf
15. Martínez Rodríguez IM. Caracterización genotípica de Mycobacterium Tuberculosis en Cuba en 2009 por MIRU – VNTR- 24. [Tesis de Máster en Bacteriología Micología]. La Habana: Instituto de Medicina Tropical Pedro Kouri; 2013.
16. Haeili M, Darban-Sarokhalil D, Imani Fooladi AA, Javadpour S, Hashemi A, Siavoshi F, et al. Spoligotyping and drug resistance patterns of Mycobacterium tuberculosis isolates from five provinces of Iran. Microbiology Open. 2013; 2(6): 988–96. DOI: 10.1002/mbo3.139
17. Cerezo-Cortés MI, Rodríguez-Castillo JG, Hernández-Pandob R, Murcia MI. Circulation of M. tuberculosis Beijing genotype in Latin America and the Caribbean. Pathogens and Global Health. 2019; 113(8): 336–51. DOI: 10.1080/20477724.2019.1710066
18. Mizukoshi F, Kobayashi N, Kirikae F, Ohta K, Tsuyuguchi K, Yamada N, et al. Molecular epidemiology of drug resistant Mycobacterium tuberculosis in Japan. mSphere. 2021; 6(4): e00978-20. DOI: 10.1128/mSphere.00978-20
19. Chung-Delgado K, Guillen-Bravo S, Revilla-Montag A, Bernabe-Ortiz A. Mortality among MDR-TB Cases: Comparison with Drug-Susceptible Tuberculosis and Associated Factors. Plos One. 2015; 10(3): e0119332. DOI: 10.1371/journal.pone.0119332
20. Battista Miglioria G, Tiberi S, Zumlad A, Petersene E, Muhwa Chakayah J, Wejsej C, et al. MDR/XDR-TB management of patients and contacts: Challenges facing the new decade. The 2020 clinical update by the Global Tuberculosis Network. International Journal of Infectious Diseases. 2020; 92S:S15-S25. DOI: 10.1016/j.ijid.2020.01.042
21. Soto Cabezas MG, Munayco Escate CV, Escalante Maldonado O, Valencia Torres E, Arica Gutiérrez J, Yagui Moscoso MJA. Perfil epidemiológico de la tuberculosis extensivamente resistente en el Perú, 2013-2015. Rev Panam Salud Publica. 2020; 44: e29. DOI: 10.26633/RPSP.2020.29
22. Lönnroth K, Mor Z, Erkens C, Bruchfeld R, Nathavitharana R, van der Werf MJ, et al. Tuberculosis in migrants in low-incidence countries: epidemiology and intervention entry points. Int J Tuberc Lung Dis. 2017; 21(6):624–36. DOI: 10.5588/ijtld.16.0845
Published
2022-08-27
How to Cite
1.
Martínez Rodríguez IM, Diaz Rodriguez R, Lemus Molina D, Rodriguez Bertheau AM, Aguilar Rojas K. Genotypic characterization with spacer oligonucleotides of multidrug-resistant isolates of Mycobacterium tuberculosis in Cuba. Rev Cubana Med Milit [Internet]. 2022 Aug. 27 [cited 2025 Jan. 8];51(3):e02202039. Available from: https://revmedmilitar.sld.cu/index.php/mil/article/view/2039
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